ABSTRACT
Objective: The aim of this study is to evaluate the impact of glucose-6- phosphate dehydrogenase deficiency in Chinese on the intracellular glucose-6-phosphate dehydrogenase activity and reactive oxygen intermediates production of peripheral neutrophil. Methods: Glucose-6-phosphate dehydrogenase, its mRNA expression and reactive oxygen intermediates production(NBT test) in silent or stimulated neutrophil from health controls and patients with glucose-6-phosphate dehydrogenase deficiency were analysized. Results: Compared with health controls, the activities of glucose-6-phosphate dehydrogenase of peripheral neutrophil from patients with glucose-6 -phosphate dehydrogenase deficiency was decreased, and its mRNA expression increased. Reactive oxygen intermediate production increased(no statistical signification) in silence but decreased following stimulation by LPS in neutrophil from normal individuals than that from patients. Conclusion: Decreased activity of G-6-PD and production of reactive oxygen intermediates following stimulation in neutrophil could be caused by G-6-PD deficiency in Chinese.
ABSTRACT
Objective: To investigate the influence of dehydroepiandrosterone(DHEA) on antioxidant capacity of Raji cell cultured in vitro. Methods: Raji cell line was cultured in vitro in the presence of DHEA and/or phenazine methosulfate(PMS), some antioxidant enzymes including glucose-6-phosphate dehydrogenase activity(G-6-PD),glutathione peroxidase activity(GPX), glutathione reductase activity(GR) and intracellular glutathione(GSH) concentration of the DHEA pretreatment cells were evaluated. meanwhile, these results were compared with that from the respective control cells. Results:(G-6-PD) activities were suppressed significantly and no changes in activities of GPX,GR and intracellular GSH concentration were occurred while Raji cells were cultured in the presence of DHEA at concentration more than 5.0?mol/L . On the other hand, the DHEA pretreatment cells show no changes in (G-6-PD,)GPX,GR activities and significantly decreased GSH concentration(P